Enzyme Inhibition & Enzyme Application in Food Processing

Enzyme inhibition is the hindrance of the enzyme activity by an enzyme inhibitor. An inhibitor is a substance, other than the enzyme, that attaches itself onto the enzyme thereby inhibiting the enzyme affinity to the substrate. The inhibitor attaches itself to the binding site of the enzyme rendering the enzyme incompatible to the substrate since the enzymes are specific to the substrate onto which they bind to catalyze metabolic processes.

#Enzyme inhibition is the hindrance of the enzyme activity by an enzyme #inhibitor Click To Tweet

Enzyme inhibition can lead to allergies when exposed to certain types of foods. The inability to produce the right enzyme for substrate metabolism may lead to complex problems such as lactose intolerance.

There are three types of enzyme inhibition namely:

1. Competitive Enzyme Inhibition

In this type of inhibition, the inhibitor binds itself to the catalytic site and competes with the substrate for the same binding site. The inhibitor has structural similarity to the substrate. It reduces enzymes by forming Enzyme-Inhibitor complex and consequently;

Vmax remains unchanged since at high levels of substrate concentration [S], all the inhibitor is displaced by the enzyme

Km is increased since higher substrate concentrations are required to reach maximum velocity

Competitive inhibition: inhibitor binds itself to the catalytic site and competes with the substrate Click To Tweet
competitive inhibition

2. Non-competitive Enzyme Inhibition

The inhibitor binds to both the Enzyme [E] and the Enzyme-Substrate [ES] complex. The inhibitor need not to be structurally similar to the substrate. As a result of the action of this type of inhibitor, we make these two observations:

Vmax reduces since, at high substrate concentration levels, the inhibitor is still bound

Km increases since higher substrate concentration [S] is necessary to reach the lower maximum velocity

non-competitive inhibition

3. Uncompetitive Enzyme Inhibition

Here, the inhibitor binds directly to the Enzyme-Substrate [ES] complex and not necessarily at the active binding site. The attachment distorts the physical conformation of the enzyme making it unfit for its substrate hence reducing the enzyme’s affinity to the substrate. The inhibitor does not necessarily have to structurally resemble the substrate.

In this type of inhibition, the Vmax is reduced since the amount of ESI formed depends on the concentration of the inhibitor.

uncompetitive inhibition

Application of Endogenous and Exogenous Enzymes in Food Processing

Enzyme application in food processing has become industry standard. Endogenous enzymes are critical in food processing. Let us consider an example of each category of enzymes to discuss the enzyme application in fruits and vegetables processing.

Endogenous enzymes

Endogenous enzymes naturally exist in food and may affect the quality of the foods (either positively or negatively) by their actions. One of the most cognitive endoenzyme is pectin methyl esterase (PME), which is found in fruits and vegetables. It affects the quality of the final products (such as fruit juices) made from these fruits and vegetables.

Endogenous enzyme application in food processing

This enzyme is applied in texture modification of the thermo-processed fruits and vegetables. This is done by the manipulation of the enzyme using the temperature. For instance, under cold break tomato juice processing, PME rapidly hydrolyses the pectin esters to form methanol, which lowers the pH of the product.

a) Cold break processes

Cold break processing involves heating the tomato to 60°C to activate the PME. The activated enzyme depolymerizes pectin leading to loss of viscosity and cloudiness in the product. This facilitates separation of solid from liquid components of the juice making the process suitable for manufacturing juices such as tomato juice.

b) Hot break processes

Hot break processing on the other hand involves heat inactivation of the PME by heating the juice to 90°C. This results in preservation of the pectin, which promotes and stabilizes cloud and viscosity of the product. Hot break processing is utilized in manufacturing of pastes and gels, e.g. tomato paste.

Exogenous enzymes

Exogenous enzymes (exo-enzymes) are those enzymes that you add into the food to promote a desirable effect. An example of the exogenous enzyme is pectin esterase; a pectinase that cracks specific bonds in molecules of pectin.

Exogenous enzyme application in food processing

Pectinase (pectin esterase) is very useful in the manufacturing of fruits and vegetables; it breaks pectin. This results into modification of the texture of fruit juices. For this reason, you can use this enzyme in thinning of pastes/juice concentration and to clarify wines.

Pectin esterase clarifies wine by breaking pectin #clarifiedwine #wine #enzymes Click To Tweet

You may also add pectin esterase during processing to not only increase the yield of fruit juice concentrates but also to gelate the products. Gelation helps in texture modification and improvement of rheological properties of these products.

Biochemical Reactions in Food

The key determinants of food quality are established to achieve the high quality standards that customers expect.

Consequently, there is need to implement measures that will ensure that there exists an environment for continuous improvement.

Every consumer expects that the food products s/he uses will not bring any harm to her/his health and that no economic injury will arise from the use of such products.

Food safety means that the food is free from any harmful physical, chemical, or any microbial contamination at the point of consumption.

Food safety is used in its operational sense e.g. in the canning industry where commercial sterility as applied in low acidity foods is taken to mean the absence of Clostridium botulinum.

To achieve this, there is need to translate the conditions for commercial sterility into a set of heating conditions for a given product in a given package.

Optimization of other quality attributes is only achieved after having first considered the basic aspect of safety.

The key determinants of food quality are propped on several factors such as:

a) The sensory appeal

Involves features such as the color, the texture, the taste, the smell, etc. of the food sample under consideration.

These are the immediate quality features that an individual recognizes when evaluating a food sample because they are easily perceptible.

An individual is more likely to make a decision on whether to use the food item or not depending on the physically perceptible quality features.

There could be undesirable changes in the texture such as the loss of solubility, loss of water holding capacity, hardening or softening.

The food product may also develop a rancid flavor or any other undesirable off-flavors that may not be very appealing to the consumer.

Changes in color such as darkening, browning or bleaching may not be very desirable in some products that are associated with a specific color.

Sensory appeal thus forms the basis for evaluation and preference by consumers.

b) The nutritive value

Considers the types and contents of nutrients contained in any given food item. It involves a methodical investigation and may require advanced equipment to perform profiled tests to determine the contents of the nutrients.

This method also includes investigation of the microbial load in the food item as well as the availability of the nutrients profiled and their digestibility.

Some foods may have certain nutrients in abundance but such nutrients remain largely unavailable for uptake by the human digestive system due to degradation.

Storage and processing conditions may lead to loss of certain vitamins, proteins and minerals.

c) Convenience and the technological quality

Facilitates handling of the food and makes the food items more functional to the manufacturer and the users.

Some of the features that enhance convenience of food items include:

  • Shelf life
  • Level of processing
  • Price – quality relation
  • Ease of storage and transportation
  • Durability
  • Dimensions of the product
  • Functionality/usefulness in the manufacturing of other products.

Some of the chemical and biochemical reactions that may compromise food quality include:

1. Enzymatic browning

Enzymatic browning occurs due to the action of polyphenoloxidase enzyme, which brings about the brown coloration in fruits, seafood, and vegetables.

The effect of enzymatic browning may lead to reduction in the quality of foods, especially during the post-harvest storage time.

Polyphenols are very unstable; therefore, they react with other components in the food to bring about the brown color effect in foods. The reactions are mostly initiated by injuries to the surface of the food sample.

SourcePhenolic substrates
Applechlorogenic acid (flesh), catechol, catechin (peel), caffeic acid, 3,4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxy benzoic acid, p-cresol, 4-methyl catechol, leucocyanidin, p-coumaric acid, flavonol glycosides
Apricotisochlorogenic acid, caffeic acid, 4-methyl catechol, chlorogenic acid, catechin, epicatechin, pyrogallol, catechol, flavonols, p-coumaric acid derivatives
Avocado4-methyl catechol, dopamine, pyrogallol, catechol, chlorogenic acid, caffeic acid, DOPA
Banana3,4-dihydroxyphenylethylamine (Dopamine), leucodelphinidin, leucocyanidin
Cacaocatechins, leucoanthocyanidins, anthocyanins, complex tannins
Coffee beanschlorogenic acid, caffeic acid
Eggplantchlorogenic acid, caffeic acid, coumaric acid, cinnamic acid derivatives
Grapecatechin, chlorogenic acid, catechol, caffeic acid, DOPA, tannins, flavonols, protocatechuic acid, resorcinol, hydroquinone, phenol
Lettucetyrosine, caffeic acid, chlorogenic acid derivatives
Lobstertyrosine
Mangodopamine-HCl, 4-methyl catechol, caffeic acid, catechol, catechin, chlorogenic acid, tyrosine, DOPA, p-cresol
Mushroomtyrosine, catechol, DOPA, dopamine, adrenaline, noradrenaline
Peachchlorogenic acid, pyrogallol, 4-methyl catechol, catechol, caffeic acid, gallic acid, catechin, dopamine
Pearchlorogenic acid, catechol, catechin, caffeic acid, DOPA, 3,4-dihydroxy benzoic acid, p-cresol
Plumchlorogenic acid, catechin, caffeic acid, catechol, DOPA
Potatochlorogenic acid, caffeic acid, catechol, DOPA, p-cresol, p-hydroxyphenyl propionic acid, p-hydroxyphenyl pyruvic acid, m-cresol
Shrimptyrosine
Sweet potatochlorogenic acid, caffeic acid, caffeylamide
Teaflavanols, catechins, tannins, cinnamic acid derivatives
Common polyphenols that cause browning (source, www.food-info.net/uk/colour/enzymaticbrowning.htm)

To prevent enzymatic browning, you can use the following methods

a) Blanching: – short heat treatment to inactivate the enzymes followed by freezing to preserve the quality of the product.

Different enzymes have different inactivation temperatures.

EnzymeEffect causedInactivation temp. (° C)
Lipolityc acyl hydrolaserancidity~ 75
Lipoxygenaserancidity~ 80
Polyphenoloxidasebrowning~100
Peroxidasedeterioration~135
Approximated inactivation temperatures of some enzymes

b) Refrigeration and chilling: – storing foods at low temperatures inhibits the enzymes responsible for the browning effect

c) Increasing the acidity of the food: – the low pH is not conducive for the enzyme activity.

d) Dehydration: – the enzymes need sufficient amount of water to work; removing water impedes their capacity to work.

e) Irradiation: – the ionized treatment reduces enzyme activity. However, irradiation may lead to loss of nutrients.

f) Pressurization: – High Pressure Processing leads to enzyme destabilization hence inactivation.

g) Ultra filtration: – will remove large components like polyphenols from the food sample.

h) Treatment with supercritical carbon dioxide: – pressurized liquid carbon dioxide is applied to the food. The resultant carbonic acid lowers the pH of the medium and impedes the activity of the enzymes.

Inhibitors of enzymatic browning

i) Reducing agents such as ascorbic acid, analogs of cysteine, glutathione, and sulphiting agents act by removing oxygen from the medium.

ii) Chelating agents such as phosphates, Ethylenediaminetetraacetic acid (EDTA) and organic acids act by removing metals since most polyphenol oxidase enzymes are metals.

iii) Acidulants such as citric acid and phosphoric acid act by lowering medium pH.

iv) Enzyme inhibitors such as aromatic carboxylic acids, peptides, and substituted resorcinols react with the enzymes.

2. Non-enzymatic browning

A form of browning effect in foods that is initiated by either Caramelization or Millard reaction. Caramelization happens when the sugars in foods are exposed to high temperatures. Water is removed from the sugar whose isomers are them polymerized.

Millard reaction occurs when the amino acids react with reducing sugars in the presence of heat (can also happen in the absence of heat).

Here, the carbonyl group of the sugar reacts with the amino group in the amino acid to produce melanoidins responsible for various flavors and colors.

Different amino acids produce different flavor components, which makes Millard reaction a key component in the flavoring industry.

Ascorbic acid and metapolyphenols can also contribute to non-enzymatic browning.

Heating leads to formation of furfurals (isomers), which then polymerize to produce the brown color observed as the browning effect.

Millard reactions may reduce the nutritive value of foods. For instance, toasting bread may lead to loss of up to half the protein efficiency ratio of bread.

3. Lipid hydrolysis

Lipid hydrolysis is the breakdown of lipids by addition of water to produce glycerol and fatty acids. This usually happens in an enzyme (lipolytic enzymes such as lipases) mediated process.

The free fatty acids produced by the hydrolysis process may lead to rancidity, which is not desirable in most cases, as the resultant food product will have a poor sensory appeal.

If the hydrolysis occurs in an aqueous sodium hydroxide medium, soap is formed.

Foods most susceptible to hydrolysis include edible oils, margarine, butter, fried foods (potato chips), roasted nuts, dried soups, broths, seasonings, milk, dried meat, frozen fish, etc.

Store foods in a cool and dry place to avoid non-enzymatic browning. Eliminate all lipases to avoid the enzymatic action on the food product.

4. Lipid oxidation

The presence of oxygen in lipid foods will initiate an oxidation process. When the oxidation process is initiated, it will lead to deterioration of the fat phase of the food.

Deterioration of fatty foods is a phenomenon commonly referred to as rancidity.

Uncontrolled oxidation of lipids could lead to formation of several cytotoxic and mutagenic compounds that are harmful to the body.

However, not all oxidative reactions in lipids are bad since the β-oxidation process utilizes lipids to produce energy.

Eicosanoids, which are signaling substances in the body, are also produced through lipid oxidation.

5. Protein denaturation

Involves the alteration of the normal protein structure and a possible destruction of the secondary conformations of such structures.

Some protein denaturation processes are irreversible e.g. cooking an egg. Heat, alcohol, heavy metal salts, acids and bases are some of the agents of protein denaturation.

6. Protein cross-linking

Protein cross-linking occurs when the reactive ends of two or more functional groups (such as amines and sulfhydrils) on proteins covalently join to form two or more biomolecules.

The resultant biomolecules may exhibit increased/reduced solubility, stability, aggregation, etc and may cause food poisoning when consumed.

7. Protein hydrolysis

Protein hydrolysis involves breakdown of proteins into its constituent amino acids through enzymatic and chemical methods. The most common chemical for protein hydrolysis hydrochloric acid while most enzymes used for protein hydrolysis are derived from animal (e.g. pepsin) and plant (e.g. ficin from fig tree) sources.

After hydrolysis, the resultant peptides and amino acids may form poisonous substances rendering the food unsafe for consumption.

8. Pigment degradation

9. Oligosaccharide and polysaccharide hydrolysis

10. Polysaccharide synthesis

11. Glycolytic changes

12. Nitrocession of amines

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